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Addendum II: Terms and Conditions for Confined Research Field Trials of Brown Mustard (Brassica juncea L.)

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Any release of a plant with a novel trait (PNT) into the environment in the absence of explicit authorization by the Director of the Plant Production Division, CFIA, is a contravention of Part V of the Seeds Regulations. Failure of the applicant to comply with conditions of an authorized release is a contravention of Part V of the Seeds Regulations.


1. Seed and plant material that is to be planted, any excess from transplantation, and that from harvesting must be transported in clearly labelled and secure containers that are to be kept separate from other seed and plant material. These requirements also apply to all non-modified plant material from the trial site.

Reproductive Isolation

2. B. juncea plants in the trial must be reproductively isolated from Brassica species by: (a) a minimum 200 metre isolation distance; or (b) growth in cages or bags. Should the isolation method (b) fails a 200 metre isolation distance from Brassica species will be required. Brassica species include: Brassica napus (Argentine rape, swede rape), Brassica rapa (oilseed rape, Polish rape canola, turnip, bird rape), Brassica juncea (brown mustard, Indian mustard), Brassica carinata (Ethiopian mustard), Brassica nigra (black mustard), Brassica hirta [also known as Sinapis alba (white mustard)], Brassica oleracea (cabbage, cauliflower, brussel sprouts, broccoli, Chinese cabbage, kale, kohlrabi). All listed Brassica species that are not part of the trials must be removed before seed set when found on the trial site and isolation distance, if used, during the year of the trial. These Brassica species must also be removed from the trial site before flowering when found during the five post-trial growing seasons.

3. Trial plants must be reproductively isolated from other related species by a minimum of 50 metres surrounding the trial site (including ditches, shelterbelts and neighbouring land), unless other methods of reproductive isolation are being used (e.g. cages or bags) without failure. The related species include: Diplotaxis muralis (sand rocket, stinking wall rocket), Raphanus raphanistrum (wild radish), and Erucastrum gallicum (dog mustard). During the year of the trial, all listed related species found on the trial site and within 50 metres surrounding the trial site, must be removed before seed set. During the three post-harvest growing seasons, these related species must be removed from the trial site before flowering.

4. The trial site, and a minimum 10 metre zone (50 metres if a commercial-scale combine was used during harvest) around the trial site, must not be seeded to Brassica species for five years following harvest of the trial (Please see section "Information to be Provided to the PBO (Plant Biosafety Office)").

Cleaning of Equipment

5. Machinery and equipment used for seeding, transplanting, site maintenance and harvesting must be cleaned of all seed and residual plant material at the trial site prior to being moved to other locations to prevent dispersal of novel plant material.

Trial Boundary Marking

6. The use of markers is strongly recommended. Markers should be placed at all corners of the trial site to identify the confined research field trial boundaries. The markers (e.g. flags, corner posts, etc.) should be obvious, identifiable and in place for the growing seasons of both the trial and post-harvest restriction period.

7. Distance measurements from permanent surrounding landmarks must be provided for precise location of the trial site. Global Positioning System (GPS) coordinates must be taken precisely at all corners of each trial site. The GPS coordinates of each confined research field trial site location must be submitted to the PBO within seven (7) days after planting.

Harvesting and Seed Set

8. It is strongly encouraged that plants be harvested before full maturity to minimize shattering and seed dispersal. If seed set occurs, all seed and other propagable plant material from the confined research field trial must be harvested unless otherwise approved by the PBO (Please see section "Information to be Provided to the PBO").


9. The following plant material that is not to be retained must be disposed of by crushing, burning autoclaving or burial at a depth of one metre: surplus seed or seedlings, plant material remaining after planting or transplantation and propagable plant material harvested from the trial site. When crushing is used to dispose of the seed, the crushed seed must be soil incorporated within 48 hours after harvest and the mechanical rollers must be thoroughly cleaned at the trial site. Regardless of destruction method, all seed must be devitalized. Composting of this material is not an acceptable destruction method. These requirements apply to all modified and non-modified plant material on the trial site.

10. All non-propagable plant material remaining on the trial site must be either soil incorporated or destroyed by incineration as soon as possible after harvest. Applicants are encouraged to destroy all non-propagable plant material in a manner whereby the material is not easily distributed by wind or local fauna, yet does not promote seed dormancy. If the applicant decides to burn the plant material, the material must be burned to ash.

11. If a trial is terminated prior to harvest, all plant material must be disposed of as described earlier in this section.


12. All retained seed and plant material, including the excess from planting and harvesting, must be stored appropriately in clearly labelled and secure containers. These containers must be kept separate from other seed and plant material. These requirements also apply to non-modified seed and plant material harvested from trial sites. A secure container must be designed to minimize the potential for a spill or dissemination. Each container must be labelled "PNT material – Do not mix". In the event of any accidental spill or dissemination of material, the PBO must be immediately notified.


13. During the trial growing season, the trial site and the isolation distance must be monitored weekly to ensure that all related species are removed before seed set.

14. During the five post-trial growing seasons the trial site and the 10 metre zone (50 metre if a commercial-scale combine was used during harvest), is subject to monitoring (Please see section "Reproductive Isolation"). Site monitoring must occur at least once every two weeks to ensure that all volunteer plants and related species are removed before flowering.

Accidental Release

15. In the case of accidental release of propagable material in the environment, recoverable seeds or seedlings must be collected and destroyed, the site must be marked and monitored, and the PBO notified immediately. Plants from unrecoverable seed must be immediately destroyed (this can include mechanical or chemical means).


16. A detailed trial log book must be kept. Records of the confined research field trial, including current season and post-harvest site monitoring, activities related to the trial site compliance, cleaning of machinery, transportation, disposition and storage of all surplus seed and harvested seed and plant material, must be maintained by the applicant and made available to the CFIA upon request. A report summarizing the completed trial and experimental data, including any amendments to the original protocol, must also be made available to the CFIA upon request. Detailed records requirements can be found in section 3.7 of Directive 2000-07.

Information to be provided to the PBO (Plant Biosafety Office)

17. In the event of any accidental release of regulated material into the environment, the PBO must be notified immediately.

18. a) Applicants must provide the following information to the PBO in writing within 15 working days after harvest:

b) If a trial is terminated prior to harvest, applicants must provide information on the trial's growth stage at the time of destruction, as well as the date and method of destruction, to the PBO in writing within 15 working days.

19. Applicants must notify the PBO in writing of the crop species planted on field trial sites for each year the sites are subject to post-harvest land use restrictions. This notification must be received every year by June 15.


20. It is not permitted to allow livestock access to sites under post-harvest restrictions. No plant material from these trials may enter the human food or livestock feed chain unless approved by Health Canada or the Animal Feed Division, CFIA, respectively.

21. If a chemical treatment is used on the trial site that requires a temporary prohibition on entry into the site, a sign must be posted at the access to the trial indicating the date and time of spraying as well as the time until safe entry. This condition is intended to protect the health and safety of the CFIA inspection staff.

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