Bacterial Pathogens and Indicators, Viruses and Parasites in Various Food Commodities – April 1, 2018 to March 31, 2022

Food microbiology – Targeted surveys – Interim report

Summary

From April 1, 2018 to March 31, 2022, a total of 18,811 food samples were tested in a suite of ongoing targeted surveysFootnote 1 to investigate the presence of bacterial pathogens and indicators, viruses and parasites.

Of the samples tested, 99.2% were satisfactory, 0.8% were investigative, and 0.06% were unsatisfactory. The Canadian Food Inspection Agency (CFIA) conducted appropriate follow-up activities including the oversight of industry led voluntary food recalls. There were no reported illnesses related to these products.

Final reports, which will include a detailed analysis of the results, will be published over the next few years as the surveys are completed.

What is an interim report

An interim report provides preliminary results related to on-going targeted surveysFootnote 1 which can vary in duration from several months to several years depending on the objective of each survey.

Will final reports be published

Yes, upon conclusion of the surveys, final reports or scientific publications will be published.

What foods were tested

The presence of bacterial pathogens and indicators, viruses and parasites were investigated in a variety of foods of the following commodities:

  • raw and pasteurized cheese
  • non-cow dairy milk
  • fresh and frozen fruits and vegetables
  • plant-based foods
  • seafood
  • powdered infant formula and baby food
  • tahini
  • guacamole
  • fresh herb paste
  • raw shelled nuts
  • ready-to-eat (RTE) sausages

Why were these foods tested

The selected foods are all commonly consumed by Canadians of all agesFootnote 2. Unfortunately, most of these commodities have been associated with recalls and outbreaks of foodborne illnesses. Contamination with pathogens can occur at any step in the food supply chain such as during production, processing, packaging, distribution and/or at retail. Given that most of the commodities covered by this report are consumed without further preparation, the presence of pathogens creates the potential for foodborne illness.

When were the samples collected

The samples were collected from April 1, 2018 to March 31, 2022.

Where were the samples collected from

Samples were collected from national retail chains and local/regional grocery stores located in 11 major cities across Canada:

  • Halifax
  • Saint John or Moncton
  • Quebec City
  • Montreal
  • Toronto
  • Ottawa
  • Vancouver
  • Kelowna or Victoria
  • Calgary
  • Saskatoon
  • Winnipeg

A sample consisted of a single or multiple consumer-size packages from the same lot weighing at least 250 g. The planned number of samples to be collected from each city was based on the population of the province in which the city was located relative to the total population of Canada.

What methods were used to test the samples

Samples were analyzed using analytical methods published in Health Canada's Compendium of Analytical Methods for the Microbiological Analysis of FoodsFootnote 3, CFIA internally-developed methods, methods from other organizations such as ISO or FDA BAM, and methods that detect the presence of deoxyribonucleic acid (DNA) of Cyclospora and Giardia.

How were the samples assessed

The samples were assessed using criteria (tables 1, 2, 3) based on the principles of the following Health Canada documents: Health Products and Food Branch (HPFB) Standards and Guidelines for Microbiological Safety of Food – An Interpretive SummaryFootnote 4, Policy on Listeria monocytogenes in Ready-to-Eat FoodsFootnote 5 and the Food and Drugs Act (Section 4 (1))Footnote 6. In the absence of Health Canada standards, policies or guidelines, the assessment criteria are based on guidelines developed by international food safety authorities. Detailed information about the assessment criteria used for each commodity will be provided in the final reports which will be published after the surveys have concluded.

Table 1. Assessment criteria for indicator organisms
Analysis Product(s) Satisfactory assessment Investigative assessment Unsatisfactory assessment
Aerobic Colony Count (ACC) Non-cow dairy milk ≤106 CFU/mL >106 CFU/mL Not Applicable (N/A)
Total coliforms Non-cow dairy milk ≤103 CFU/mL >103 CFU/mL N/A
Generic Escherichia coli (E. coli) Non-cow pasteurized dairy milk cheese ≤ 102 CFU or MPN/g >102 and ≤2×103 CFU or MPN/g >2×103 CFU or MPN/g
Generic E. coli Fresh fruits and vegetables ≤ 102 CFU/g or MPN/g >102 and ≤103 CFU/g or MPN/g >103 CFU/g or MPN/g
Generic E. coli RTE plant-based meat ≤ 102 CFU/g >102 and ≤103 CFU/g >103 CFU/g
Generic E. coli Raw plant-based meat ≤102 CFU/g > 102 CFU/g N/A
Generic E. coli Arctic surf clams ≤4 MPN/g > 4 and ≤ 40 MPN/g >40 MPN/g
Generic E. coli Seaweed salad ≤102 MPN/g > 102 MPN/g N/A
Generic E. coli Raw dairy milk cheese ≤5×102 CFU or MPN/g >5×102 and ≤2×103 CFU or MPN/g >2×103 CFU or MPN/g
Table 2. Assessment criteria for pathogenic bacteria
Analysis Product(s) Satisfactory assessment Investigative assessment Unsatisfactory assessment
E. coli O157 All products tested for E. coli O157 Not detected/25 g N/A Detected/25 g
Non-O157 Shiga-toxigenic E. coli (STEC) All products tested for non-O157 STEC Not detected/25 g Detected/25 g N/A
Listeria monocytogenes (L. monocytogenes) Category 1 productsTable Note a Not detected/25 g N/A Detected/25 g
L. monocytogenes Category 2A and 2B productsTable Note a Not detected/25 g ≤102 CFU/g >102 CFU/g
L. monocytogenes Microgreens
Whole mushrooms
Whole fruits
Not detected/25 g Detected/25 g (enumeration is required) N/A
Salmonella All products tested for Salmonella (except for specified products) Not detected/25 g N/A Detected/25 g
Salmonella RTE meat sausages Not detected/65 g N/A Detected/65 g
Salmonella Powdered infant formula Not detected/125 g N/A Detected/125 g
Staphylococcus aureus (S. aureus) Plant-based RTE meat ≤104 CFU/g >104 CFU/g N/A
S. aureus Raw dairy milk cheese ≤103 CFU/g >103 and ≤ 104 CFU/g >104 CFU/g
S. aureus Non-cow pasteurized dairy milk cheese ≤102 CFU/g >102 and ≤104 CFU/g >104 CFU/g
S. aureus Ready-to-eat meat sausages ≤250 CFU/g >250 and ≤104 CFU/g >104 CFU/g
Enterobacteriaceae Powdered infant formula Not detected/10 g Detected/10 g N/A
Cronobacter Powdered infant formula Not detected/125 g N/A Detected/125 g
Commercial sterility Baby foods in retort pouches Pass N/A Fail
Table 3. Assessment criteria for viruses and parasites
Analysis Product(s) Satisfactory assessment Investigative assessment Unsatisfactory assessment

CyclosporaTable Note b

GiardiaTable Note b

Hepatitis A Virus (HAV)Table Note b

Norovirus Genotype (I and II) (NoV (GI and GII))Table Note b

All products tested for parasites or viruses Not detected Detected N/A

What were the survey preliminary results

Details about each targeted survey and test results (as of March 31, 2022) can be found in table 4.

Table 4. Survey results as of March 31, 2022
Fiscal year(s) Product
(sample plan ID)
Microorganisms tested Number of samples tested Satisfactory (S) Investigative (I) Unsatisfactory (U) Comments
2020/2021
2021/2022
Imported raw dairy milk cheese
(SB3015)
L. monocytogenes Salmonella
E. coli O157
S. aureus
Generic E. coli
784 784 0 0 All satisfactory
2021/2022 Domestic raw dairy milk cheese
(SB3098)
L. monocytogenes Salmonella
E. coli O157
S. aureus
Generic E. coli
299 299 0 0 All satisfactory
2021/2022 Non-cow raw milk cheese
(SB3097)
L. monocytogenes Salmonella
S. aureus
Generic E. coli
288 287 0 1 U=Generic E. coli
2019/2020
2020/2021
2021/2022
Non-cow pasteurized dairy milk cheese
(SB3077)
L. monocytogenes Salmonella
S. aureus
Generic E. coli
616 615 1 0 I=Generic E. coli
2019/2020
2020/2021
2021/2022
Non-cow dairy milk
(SB3073)
L. monocytogenes Salmonella
ACC
Total coliforms
506 434 72 0 I=ACC (59)
I=Total coliforms (4)
I=ACC & total coliforms (9)
2019/2020
2020/2021
2021/2022
Fresh seed sprouts
(SB3082)
Salmonella (2021-2022 only)
E. coli O157
STEC
Generic E. coli
709 706 2 1 I=VTEC(1)
I=Generic E. coli (1)
U=Generic E. coli
2019/2020 Fresh microgreens
(SB3083)
Salmonella
E. coli O157
STEC
Generic E. coli
L. monocytogenes
75 73 2 0 I=L. monocytogenes
I=Generic E. coli
2021/2022 Fresh whole mushrooms (enoki, beech, oyster, king oyster)
(SB3114)
L. monocytogenes
Salmonella
E. coli O157
Generic E. coli
493 484 9 0 I=L. monocytogenes (9)
2021/2022 Red onions
(SB3115)
Salmonella
E. coli O157
Generic E. coli
300 300 0 0 All satisfactory
2021/2022 Imported green onions
(SB3223)
HAV
NoV (GI, GII)
199 199 0 N/A All satisfactory
2021/2022 Domestic green onions
(SB3224)
HAV
NoV (GI, GII)
54 54 0 N/A All satisfactory
2020/2021
2021/2022
Domestic whole leafy vegetables
(SB3092)
Salmonella (2021-2022 only)
E. coli O157
VTEC
Generic E. coli
245 243 2 0 I=Generic E. coli (2)
2021/2022 Domestic whole leafy vegetables
(SB3102)
Salmonella
E. coli O157
Generic E. coli
193 192 1 0 I=Generic E. coli (1)
2021/2022 Domestic fresh whole vegetables
(SB3106)
Salmonella
E. coli O157
Generic E. coli
300 300 0 0 All satisfactory
2020/2021
2021/2022
Imported whole leafy vegetables
(SB3093)
Salmonella (2021-2022 only)
E. coli O157
VTEC
Generic E. coli
285 284 1 0 I=VTEC
2021/2022 Imported whole leafy vegetables
(SB3101)
Salmonella
E. coli O157
Generic E. coli
283 283 0 0 All satisfactory
2021/2022 Imported fresh whole vegetables
(SB3105)
Salmonella
E. coli O157
Generic E. coli
495 494 1 0 I=Generic E. coli
2019/2020
2020/2021
2021/2022
Imported
romaine lettuce
(SB3085)
Salmonella (2021-2022 only)
E. coli O157
Generic E. coli
709 709 0 0 All satisfactory
2019/2020
2020/2021
2021/2022
Imported romaine lettuce hearts
(SB3086)
Salmonella (2021-2022 only)
E. coli O157
Generic E. coli
1059 1058 1 0 I=Generic E. coli
2021/2022 Imported fresh whole leafy vegetables
(SB3221)
Cyclospora
Giardia
179 177 2 N/A I=Cyclospora (2)
2021/2022 Imported fresh leafy herbs
(SB3103)
Salmonella
E. coli O157
Generic E. coli
389 385 3 1 I=Generic E. coli (3)
U=Generic E. coli
2021/2022 Domestic fresh leafy herbs
(SB3104)
Salmonella
E. coli O157
Generic E. coli
189 188 1 0 I= Generic E. coli (1)
2020/2021 Domestic whole fresh vegetables and herbs
(F258R)
Salmonella
E. coli O157
Generic E. coli
91 91 0 0 All satisfactory
2020/2021 Imported whole fresh vegetables and herbs
(F259R)
Salmonella
E. coli O157
Generic E. coli
196 196 0 0 All satisfactory
2020/2021 Domestic fresh-cut RTE prepackaged vegetable salads
(F242R)
L. monocytogenes Salmonella
E. coli O157
Generic E. coli
15 15 0 0 All satisfactory
2020/2021 Imported fresh-cut RTE prepackaged vegetable salads
(F247R)
L. monocytogenes Salmonella
E. coli O157
Generic E. coli
193 193 0 0 All satisfactory
2021/2022 Fresh-cut RTE prepackaged leafy vegetable- based salads with dressing kids
(SB3209)
L. monocytogenes Salmonella
E. coli O157
Generic E. coli
424 422 0 2 U=L. monocytogenes (1)
U=Salmonella (1)
2021/2022 Fresh-cut RTE prepackaged leafy vegetable-based salads without dressing kits
(SB3210)
L. monocytogenes Salmonella
E. coli O157
Generic E. coli
386 383 0 3 U=L. monocytogenes (3)
2021/2022 Imported fresh-cut leafy vegetable-based salads
(SB3217)
HAV
NoV (GI, GII)
279 279 0 N/A All satisfactory
2021/2022 Domestic fresh-cut leafy vegetable-based salads
(SB3218)
HAV
NoV (GI, GII)
105 105 0 N/A All satisfactory
2019/2020
2020/2021
2021/2022
Imported fresh-cut romaine lettuce
(SB3087)
Salmonella (2021-2022 only)
E. coli O157
Generic E. coli
797 797 0 0 All satisfactory
2020/2021
2021/2022
Imported fresh-cut leafy vegetable-based salads
(F263R &SB3222)
Cyclospora
Giardia
616 614 2 N/A I=Cyclospora
I=Giardia
2020/2021 Domestic fresh whole fruits
(F260R)
Salmonella
E. coli O157
Generic E. coli
81 81 0 0 All satisfactory
2020/2021 Imported fresh whole fruits
(F261R)
Salmonella
E. coli O157
Generic E. coli
194 194 0 0 All satisfactory
2021/2022 Imported fresh whole fruits
(SB3107)
L. monocytogenes
Salmonella
E. coli O157
Generic E. coli
397 395 2 0 I=L. monocytogenes (2)
2021/2022 Domestic fresh whole fruits
(SB3108)
L. monocytogenes
Salmonella
E. coli O157
Generic E. coli
200 197 3 0 I=L. monocytogenes (3)
2021/2022 Imported fresh berries
(SB345)
HAV
NoV (GI, GII)
298 298 0 N/A All satisfactory
2021/2022 Domestic fresh berries
(SB346)
HAV
NoV (GI, GII)
118 118 0 N/A All satisfactory
2021/2022 Fresh-cut fruits
(SB3211)
L. monocytogenes
Salmonella
E. coli O157
Generic E. coli
420 420 0 0 All satisfactory
2021/2022 Frozen berries
(SB3215)
HAV
NoV (GI, GII)
294 293 1 N/A I=Norovirus
2021/2022 Frozen cut fruits
(SB3112)
L. monocytogenes
Salmonella
E. coli O157
Generic E. coli
297 297 0 0 All satisfactory
2021/2022 Frozen cut fruits (bulk packaged)
(SB3212)
L. monocytogenes
Salmonella
E. coli O157
Generic E. coli
135 135 0 0 All satisfactory
2018/2019
2019/2020
Imported frozen- cut fruits
(SB3063)
HAV
NoV (GI, GII)
484 484 0 N/A All satisfactory
2021/2022 Frozen- cut fruits
(SB3216 & SB3088)
HAV
NoV (GI, GII)
558 558 0 N/A All satisfactory
2020/2021
2021/2022
Plant-based RTE sausages / burgers
(SB3094)
L. monocytogenes Salmonella
S. aureus
Generic E. coli
753 753 0 0 All satisfactory
2020/2021
2021/2022
Plant-based Raw sausages / burgers
(SB3095)
Salmonella
E. coli O157
Generic E. coli
487 484 3 0 I=Generic E. coli (3)
2020/2021
2021/2022
Imported tahini
(FS358R & SB3099)
Salmonella 256 253 N/A 3 U=Salmonella (3)
2021/2022 Guacamole
(SB3113)
L. monocytogenes
Salmonella
E. coli O157
Generic E. coli
299 299 0 0 All satisfactory
2021/2022 Herb paste
(SB3116)
L. monocytogenes
Salmonella
E. coli O157
Generic E. coli
28 28 0 0 All satisfactory
2021/2022 Seaweed salad
(SB3117)
L. monocytogenes
Salmonella
E. coli O157
Generic E. coli
290 290 0 0 All satisfactory
2021/2022 Domestic oysters
(SB3219)
HAV
NoV (GI, GII)
132 126 6 N/A I=NoV (GI and GII) (3)
I=Nov (GII) (3)
2021/2022 Imported oysters
(SB3220)
HAV
NoV (GI, GII)
52 23 29 N/A I=NoV (GI) (8)
I=NoV (GII) (8)
I=NoV (GI and GII) (13)
2021/2022 Arctic surf clams
(SB3118)
L. monocytogenes
Salmonella
Generic E. coli
55 55 0 0 All satisfactory
2021/2022 RTE meat sausages
(SB3213)
L. monocytogenes
Salmonella
S. aureus
Generic E. coli
285 284 1 0 I=Generic E. coli
2021/2022 Raw shelled nuts
(SB3119)
Salmonella
E. coli O157
Generic E. coli
494 494 0 0 All satisfactory
2020/2021
2021/2022
Powdered infant formula
(FS360R & SB3100)
Salmonella (2021-2022 only)
Cronobacter Enterobacteriaceae
267 266 1 0 I=Enterobacteriaceae
2021/2022 Baby food in retort pouches
(SB3214)
Commercial sterility 186 186 0 0 All satisfactory
Total (%) - - 18,811 18,654 (99.2) 146 (0.8) 11 (0.06) -

What is done with the survey results

All results are used to:

  • inform risk management decisions
  • support program design and re-design

All investigative and unsatisfactory results triggered appropriate follow-up actions such as:

  • on site visit of the manufacturer
  • follow-up with the importer
  • review of hygiene and sanitation procedures
  • review of production and distribution records
  • review of previous laboratory test results
  • additional sampling and testing
  • food recalls

Can I access the survey data

All of the results reported herein are considered preliminary, as the targeted survey are still ongoing. Therefore, the data will only be accessible to the public on the Open Government portal following the publication of the final report or scientific publication.